The distribution of 3-hydroxy-3-methylglutaryl coenzyme A reductase in the small intestine of rats fed raw or gelatinized starch as a carbohydrate source.

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The distribution of 3-hydroxy-3-methylglutaryl coenzyme A reductase in the small intestine of rats fed raw or gelatinized starch as a carbohydrate source.

The effect of gelatinization of dietary starch (wheat, corn and potato) on the distribution of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase along the small intestine of rats was studied in villus and crypt cell populations isolated by graduated scraping. Gelatinized starch preferentially stimulated jejunal reductase activity in both villus and crypt cells and consequently retained ...

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Purification of 3-hydroxy-3-methylglutaryl coenzyme A reductase.

The enzyme 3-hydroxy-3-methylglutaryl coenzyme agarose, glucose-6-phosphate dehydrogenase, and aldolA reductase from human liver was purified to homogeneity. ase were from Sigma Chemical co.; agarose-hexaneHMG-CoA was from P-L Biochemicals; bovine serum Electrophoresis of the purified human enzyme on non-denaturing gels revealed a single protein-staining band that co-migrated with reductase act...

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Reversible inactivation-reactivation of 3-hydroxy-3-methylglutaryl coenzyme A reductase of rat intestine.

3-Hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase in the ileum of rats was inactivated by Mg2+-ATP and reversibly reactivated by cytoplasmic activator from the liver. The mevalonate kinase reaction was presumably not involved in this inactivation. Studies of nucleotide specificity for the inactivation revealed that ATP was most effective in the reaction among the nucleotides tested. In ...

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The role of the 3-hydroxy 3-methylglutaryl coenzyme A reductase cytosolic domain in karmellae biogenesis.

In all cells examined, specific endoplasmic reticulum (ER) membrane arrays are induced in response to increased levels of the ER membrane protein 3-hydroxy 3-methylglutaryl coenzyme A (HMG-CoA) reductase. In yeast, expression of Hmg1p, one of two yeast HMG-CoA reductase isozymes, induces assembly of nuclear-associated ER stacks called karmellae. Understanding the features of HMG-CoA reductase t...

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The regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase in the isolated perfused rat liver.

The effect of perfusion of an isolated rat liver on hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase was studied. In liver removed during the basal period of the diurnal cycle of enzyme activity, a 227 +/- 41% increase in enzyme activity occurred after 3 h of a plasma-free perfusion. This could be prevented by the addition of cycloheximide or pure cholesterol (dispersed with lecithin) to...

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ژورنال

عنوان ژورنال: Journal of Nutritional Science and Vitaminology

سال: 1984

ISSN: 0301-4800,1881-7742

DOI: 10.3177/jnsv.30.171